At the onset of the 2022 Russian invasion of Ukraine, the brigade's elements dispersed in anticipation of the imminent barrage of artillery, rockets, and airstrikes in the opening hours. Following the bombardment, the unit returned to Chernihiv to respond to Russian forces from the 41st Combined Arms Arm which consisted of a number of meeting engagements in the surrounding forests. Russian forces made attempts to take the city on the road to Kyiv, but were repelled; they eventually bypassed the city. The brigade, augmented by Territorial Defense Forces and reserves, remained in Chernihiv while Russian reinforcements began to encircle the city as a result of more failed assault. This resulted in the Siege of Chernihiv. Despite encirclement, the brigade maintained one supply route for communications and resupply. By 31 March 2022, the brigade had successfully defended the city against Russian attacks and the siege was lifted. Surviving elements of the Russian 41st CAA retreated north to Belarus. The brigade recaptured surrounding Ukrainian towns and the M01 Highway connecting to Kyiv.
Following, the brigade was deployed to the north of the Donbas before conducting rest and refit in May. By July, elements of the brigade were deployed back to the Battle of Donbas in the area of southern Donetsk and Kramatorsk. The unit was given the recently established honorary award of "For Courage and Bravery" by Ukrainian President Volodymyr Zelenskyy for its service in Chernihiv, the southern counteroffensive, and the Donbas region. He went further stating "in six months, more than a thousand warriors of this brigade were awarded state awards
